产品名称
Anti-Heat Shock Factor II Antibody, clone 3E2, clone 3E2, Chemicon®, from rat
Quality Level
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3E2, monoclonal
species reactivity
human, monkey, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSF2(3298)
Analysis Note
Control
POSITIVE CONTROL:
NIH-3T3 cells
POSITIVE CONTROL:
NIH-3T3 cells
Application
Anti-Heat Shock Factor II Antibody, clone 3E2 is an antibody against Heat Shock Factor II for use in ELISA, IF, IP & WB.
Immunofluorescence Immunohistology (predominantly cytoplasmic with some nuclear). Immunoprecipitation: 2 μg/mg protein lysate. Western blot. 1:200-1:400 ELISA
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
MAB88079 recognizes a protein of 70 kDa, identified as Heat Shock Factor 2 (HSF2). The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediated by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken, and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosphila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. the phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Full length recombinant mouse HSF2 proteins (aa 1-517)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Nitzan Levy et al.
Endocrinology, 148(7), 3449-3458 (2007-03-31)
Estrogen receptors (ERs) regulate transcription by interacting with regulatory elements in target genes. However, known ER regulatory elements cannot explain the expression profiles of genes activated by estradiol (E2) and selective estrogen receptor modulators (SERMs). We previously showed that the
Linda Pesonen et al.
Cell stress & chaperones, 26(5), 819-833 (2021-08-01)
Cancer cells rely on heat shock proteins (HSPs) for growth and survival. Especially HSP90 has multiple client proteins and plays a critical role in malignant transformation, and therefore different types of HSP90 inhibitors are being developed. The bioactive natural compound
Marek A Budzyński et al.
Molecular and cellular biology, 35(14), 2530-2540 (2015-05-13)
In mammals the stress-inducible expression of genes encoding heat shock proteins is under the control of the heat shock transcription factor 1 (HSF1). Activation of HSF1 is a multistep process, involving trimerization, acquisition of DNA-binding and transcriptional activities, which coincide
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