Anti-GEF-H1, clone 3C5

Anti-GEF-H1, clone 3C5, Cat. No. MABC1096, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1)and has been tested for use in Western Blotting.

Western Blotting

Western Blotting Analysis: A representative lot detected GEF-H1 in Western Blotting applications (Cullis, J., et. al. (2014). Cancer Cell. 25(2):181-95).

Clone 3C5 is a mouse monoclonal antibody that specifically detects human Rho guanine nucleotide exchange factor 2 (GEF-H1).

A linear peptide from the N-terminal region of human GEF-H1

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in lysates from untreated HCT116 cells and those pretreated with MEK inhibitors PD98059 and U0126.

Western Blotting Analysis: 2 µg/mL of this antibody detected GEF-H1 in lysates from untreated HCT116 cells, but not in cells pretreated with MEK inhibitors PD98059 and U0126.

Rho guanine nucleotide exchange factor 2 (UniProt: Q92974; also known as Guanine nucleotide exchange factor H1, GEF-H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40) is encoded by the ARHGEF2 (also known as KIAA0651, LFP40) gene (Gene ID: 9181) in human. GEF-H1 is a microtubule-associated guanine nucleotide exchange factor (GEF) for the Rho family of small GTPases that activates Rho-GTPases by promoting the exchange of GDP for GTP. It localizes to the tips of cortical microtubules of the mitotic spindle during cell division and is released upon microtubule depolymerization. GEF-H1 is involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and innate immune response. GEF-H1 can act as an adaptor protein that links PP2A B subunits to KSR-1 and mediate the dephosphorylation of KSR-1 serine 392 and activate MAPK signaling. GEF-H1 I known to contributes to cell survival and growth in HRASV12- transformed cells and is reported to be essential for activation of MAP kinase pathway in response to HRASV12. It is also reported to be important for the growth and survival of HRASV12 transformed cells. GEF-H1 contains a DH (DBL-homology) domain (aa 235-432) that interacts with and promotes loading of GTP on RhoA and tyrosine phosphorylation of RIPK2. It also has two phorbol-ester/DAG-type zinc finger (aa 587-611) and the C-terminal coiled coil domains (aa 798-867), both of which are required for association with microtubules. (Ref.: Cullis, J., et al. (2014). Cancer Cell 25(2); 181-195).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.