Anti-AIM2 Antibody, clone 3B10

Quality Control Testing

Evaluated by Western Blotting in lysates from HL60 cells treated with recombinant human IFN- (100 units; 24 h).

Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected AIM2 in lysates from HL60 cells treated with recombinant human IFN- (100 units; 24 h).

Tested Applications

Western Blotting Analysis: A representative lot detected AIM2 in Western Blotting applications (Cresswell, K.S., et al. (2005). Biochem Biophys Res Commun. 326(2):417-24; Fernandes-Alnemri, T., et al. (2009). Nature. 458(7237):509-13; Vajjhala, P.R., et al. (2015). J Biol Chem. 290(49):29217-30).

Immunocytochemistry Analysis: A representative lot detected AIM2 in Immunocytochemistry applications (Cresswell, K.S., et al. (2005). Biochem Biophys Res Commun.326(2):417-24; Vajjhala, P.R., et al. (2015). J Biol Chem. 290(49):29217-30).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3B10 is a mouse monoclonal antibody that detects AIM2. It targets an epitope within the N-terminal half.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Interferon-inducible protein AIM2 (UniProt: O14862; also known as Absent in melanoma 2) is encoded by the AIM2 gene (Gene ID: 9447) in human. The human HIN-200 family proteins consist of four members known as IFI 16, MNDA, AIM2, and IFIX. AIM2 is an IFN- inducible protein that is expressed in spleen, small intestine, peripheral blood leukocytes, and testis. It is involved in innate immune response by recognizing cytosolic double-stranded DNA and induces caspase-1-activating inflammasome formation in macrophages. Its HIN-20 domain is localized in amino acids 138-337, which mediates dsDNA binding via electrostatic interactions. At its N-terminal region it contains a pyrin domain (aa 1-87) and at the C-terminal it has an oligonucleotide/oligosaccharide-binding domain that senses cytoplasmic DNA. It is reported to interact with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. It detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner that triggers its oligomerization and formation of AIM2 inflammasome. It also acts as a mediator of pyroptosis, necroptosis, and apoptosis, which are integral parts of host defense against pathogens. Clone 3B10 is shown to specifically recognize AIM2 and does not react with other related member of the HIN-200 family. (Ref.: Vajjhala, PR., et al. (2015). J. Biol. Chem. 290(49); 29217-29230; Fernandes-Alnemri, T., et al. (2009). Nature. 458(7237); 509-513; Creswell, KS., et al. (2005). Biochem. Biophys. Res. Commun. 326(2); 417-42).

GST-tagged recombinant fragment corresponding to 186 amino acids from the N-terminal half of human AIM2.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.