biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2D2-F11, monoclonal
species reactivity (predicted by homology)
all
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
isotype
IgG1κ
shipped in
ambient
target post-translational modification
unmodified
General description
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes.
Immunogen
Purified Precision Plus All Blue marker proteins.
Application
Anti-BLUE, clone 2D2-F11, HRP-conjugated, Cat. No. MABC1691H, is a mouse monoclonal antibody that detects prestained Protein marker proteins and has been tested for use in Western Blotting.
Western Blotting Analysis: 0.25 ug/mL from a representative lot detected BLUE in Nitrocellulose membrane.
Biochem/physiol Actions
Anti-BLUE, clone 2D2-F11,HRP-conjugate is a mouse monoclonal antibody that detects prestained Protein marker proteins.
Analysis Note
Evaluated by Western Blotting in PVDF membrane.
Western Blotting Analysis: 0.25 ug/mL of this antibody detected BLUE-stained marker protein standards in PVDF membrane.
Western Blotting Analysis: 0.25 ug/mL of this antibody detected BLUE-stained marker protein standards in PVDF membrane.
Other Notes
Concentration: Please refer to lot specific datasheet.
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