biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2D2-F11, monoclonal
species reactivity (predicted by homology)
all
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
isotype
IgG1κ
target post-translational modification
unmodified
Quality Level
General description
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes.
Immunogen
Prestained Precision Plus Protein marker proteins.
Application
Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins and has been tested for use in Western Blotting.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected BLUE in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).
Biochem/physiol Actions
Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Pre-Stained Protein Standard.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected BLUE-stained marker protein standards.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected BLUE-stained marker protein standards.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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