Anti-MST1R/CD136 Antibody

Macrophage-stimulating protein receptor (UniProt: Q04912; also known as EC:2.7.10.1, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron, RON, CD136) is encoded by the MST1R (also known as PTK8, RON) gene (Gene ID: 4486) in human. CD136/RON, is a single-pass type I membrane glycoprotein of the MET family of receptors, is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-957), a transmembrane domain (aa 958-978), and a cytoplasmic domain (aa 979-1400). It is expressed as a 185-kDa glycosylated pro-form, which is proteolytically processed into a 35 kDa a-chain (aa 25-304) and 150 kDa b-chain (aa 310-1400) that are linked by disulfide bridges. The a-chain is shown to be fully extracellular while the b-chain is composed of an extracellular SEMA domain, three IPT domains, a transmembrane domain and an intracellular tyrosine kinase domain. CD136/RON expression has been reported in colon, skin, lung, and bone marrow. It serves as a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to macrophage stimulating 1 (MST1) ligand and regulates many physiological processes including cell survival, migration, and differentiation. Ligand binding on tyrosine 1238 and 1239 in the kinase domain is shown to induce its autophosphorylation, which leads to further phosphorylation on tyrosine 1353 and 1360 in the C-terminal multi-functional docking site. The activated form interacts with the PI3-kinase subunit PIK3R1, PLCG1, or the adapter GAB1 and the recruitment of these downstream effectors leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, and PLC g -PKC pathways. CD136 signaling is shown to activate the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. It also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Mutations in MST1R gene are reported to cause nasopharyngeal carcinoma. Clone 3G4 binds to an epitope on the a-chain and detects a-chain and the pro form of CD136/RON. It is shown to bind strongly to deglycosylated CD136/RON in tunicamycin treated cancer cells. (Ref.: Koh, XY., et al. (2019). Oncogene. 38(48); 7342-7356).

GST-tagged recombinant fragment corresponding to 238 amino acids from the extracellular domain of human RON/MST1R/CD136.

Quality Control Testing

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected MST1R/CD136 in HT-29 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected MST1R/CD136 in Western Blotting applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

Immunocytochemistry Analysis: A representative lot detected MST1R/CD136 in Immunocytochemistry applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

Immunofluorescence Analysis: A representative lot detected MST1R/CD136 in Immunofluorescence applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

Flow Cytometry Analysis: A representative lot detected MST1R/CD136 in Flow Cytometry applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

ELISA Analysis: A representative lot detected MST1R/CD136 in ELISA applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MST1R/CD136, clone 3G4, Cat. No. MABC1782, is a mouse monoclonal antibody that detects Macrophage-stimulating protein receptor and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting.

Clone 3G4 is a mouse monoclonal antibody that detects Macrophage-stimulating protein receptor (RON/MST1R/CD136). It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.