Anti-Claudin-6 Antibody, clone 2

Claudin-6 (UniProt: P56747; also known as Skullin) is encoded by the CLDN6 (also known as UNQ757/PRO1488) gene (Gene ID: 9074) in human. Claudin-6 is a multi-pass membrane protein that plays a major role in tight junction-specific obliteration of the intercellular space. Claudin-6 has two extracellular domains (aa 29-81 and 138-160), four transmembrane domains (aa 8-28, 82-102, 117-137, and 161-181), and three cytoplasmic domains (aa 1-7; 103-116, and 182-220). It acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells. Claudin-6 is expressed in the liver, in peripheral blood mononuclear cells, and hepatocarcinoma cell lines. It is also expressed specifically in undifferentiated stem cells and can serve as a marker to purify stem-like cells from a mixed cell population. It is shown to play an important role during mouse embryonic epithelium formation and the development of endodermal tissues, and its level of expression is correlated with dedifferentiation and malignant proliferation of epithelial cells. (Ref.: Kong, F-E. et al. (2021). Sci. Transl. Med. 13, eabb6282; Wang, L., et al. (2012). Cell Res. 22; 1082-1085; Zheng, A., et al. (2007). J. Virol. 81(22); 12465-12471).

Extracellular peptides specific for human Claudin-6.

Quality Control Testing

Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Claudin-6 in NTERA-2 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Claudin-6 in Immunocytochemistry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

Inhibition Assay: A representative lot inhibited tumor growth of HepG2 xenograft tumors in a murine model. (Kong, F-E., et al. (2021). Sci Transl Med.;13(579):eabb6282).

Immunohistochemistry Applications: A representative lot detected Claudin-6 in Immunohistochemistry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

Western Blotting Analysis: A representative lot detected Claudin-6 in Western Blotting applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

Flow Cytometry Analysis: A representative lot detected Claudin-6 in Flow Cytometry applications (Kong, F-E., et al. (2021). Sci Transl Med. 13(579):eabb6282).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2 is a mouse monoclonal antibody that detects Claudin-6. It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG2a in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.