Anti-TRAF1 Antibody, clone 1F3

TNF receptor-associated factor 1 (UniProt: Q13077; also known as Epstein-Barr virus-induced protein 6, TRAF1) is encoded by the TRAF1 (also known as EB16) gene (Gene ID: 7185) in human. TRAF1 is homodimeric pro-survival adapter molecule that regulates the activation of NF-kB and JNK. It can also exist as a heterotrimer with TRAF2. It plays a role in the regulation of cell survival and apoptosis. The heterotrimer form is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. The C-terminal region of TRAF1 contains the TRAF domain (aa 266-412) that binds to receptor cytoplasmic domains. It also has a coiled coil domain (aa 182-264) that mediates its homo- and heterotrimerization. TRAF1 is also reported to essential for memory T cell survival. TRAF1 protein level is significantly reduced in HIV-specific CD8 T cells with progression of infection. Its levels can increase following treatment with IL-7. Hence, TRAF1 levels could serve as a useful parameter in monitoring effectiveness of anti-HIV therapies. (Ref.: Wang, C., et al. (2018). J. Immunol. 200(2); 558-564).

~46 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~33 kDa

GST-tagged recombinant protein corresponding to human TRAF1.

Anti-TRAF1, clone 1F3 detects levels of TRAF1 proteins & has been published & validated for use in WB, IHC & Flow.

Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in certain cell lines (Siegler, G., et al. (2003). J Clin Pathol: Mol Pathol. 56:156-161.).
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect TRAF1 in PBMCs and in certain T cells from HIV-infected individuals (Wang, C., et al. (2011). JEM.1-15.).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
B95-8cell lysate

Evaluated by Western Blot in B95-8 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected TRAF1 in 10 µg of B95-8cell lysate.

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