产品名称
抗-Atg13抗体，克隆2H4.2, clone 2H4.2, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2H4.2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ATG13(9776)
Analysis Note
对照
喜树碱处理的HeLa细胞裂解液。
喜树碱处理的HeLa细胞裂解液。
通过蛋白质印迹在喜树碱处理的HeLa细胞裂解液中进行了评估。
蛋白质印迹分析：该抗体的1:1,000稀释液在10 µg喜树碱处理的HeLa细胞裂解液中检测到Atg13。
蛋白质印迹分析：该抗体的1:1,000稀释液在10 µg喜树碱处理的HeLa细胞裂解液中检测到Atg13。
Application
研究子类别
凋亡-附加
凋亡-附加
研究类别
细胞凋亡 & 癌症
细胞凋亡 & 癌症
蛋白质印迹分析：代表性批次在HeLa细胞裂解液中检测到Atg13。
免疫组化分析：代表性批次的1:50稀释液在正常人体黑质组织中检测到Atg13。
免疫组化分析：代表性批次的1:50稀释液在正常人体黑质组织中检测到Atg13。
该抗-Atg13抗体（克隆2H4.2）经验证可用于蛋白质印迹、IHC中Atg13的检测。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
自噬相关蛋白13（Atg13）在自噬体的形成过程中发挥着重要作用。自噬体的形成是对营养匮乏的反应，可作为细胞器、蛋白质和蛋白质复合物的运输囊泡，靶向消化这些货物以产生能量和营养的溶酶体。Atg13由mTOR途径激活，并与FIP200蛋白形成复合物。该复合物参与增强ULK1激酶的活性，而ULK1激酶是自噬体形成所必需的。Atg13/FIP200可帮助ULK1定位于前自噬体，并随后稳定ULK1。自噬是发育、生长和细胞分化的一个重要过程，这一过程的破坏可能会导致癌症、衰老和神经退行性疾病。
观测值〜60 kDa
Immunogen
标记GST的重组蛋白，对应于人Atg13。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ，溶于含0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yo-Hei Yamamoto et al.
The Journal of cell biology, 219(8) (2020-06-04)
In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains
Correction: ERdj8 governs the size of autophagosomes during the formation process.
Yo-Hei Yamamoto et al.
The Journal of cell biology, 220(9) (2021-08-07)
Marianna Carinci et al.
The EMBO journal, 40(10), e103563-e103563 (2021-05-02)
The early secretory pathway and autophagy are two essential and evolutionarily conserved endomembrane processes that are finely interlinked. Although growing evidence suggests that intracellular trafficking is important for autophagosome biogenesis, the molecular regulatory network involved is still not fully defined.
Naoki Tamura et al.
Molecular and cellular biology, 39(16) (2019-06-05)
Autophagy is considered an adaptive mechanism against hyperosmotic stress. Although the process has been reported to be triggered by the inhibition of mTORC1, the precise downstream mechanisms remain elusive. Here, we demonstrate that hyperosmotic-stress-induced autophagy is different from conventional macroautophagy
Ubiquitination Is a Novel Post-Translational Modification of VMP1 in Autophagy of Human Tumor Cells.
Felipe J Renna et al.
International journal of molecular sciences, 24(16) (2023-08-26)
Autophagy is a tightly regulated catabolic process involved in the degradation and recycling of proteins and organelles. Ubiquitination plays an important role in the regulation of autophagy. Vacuole Membrane Protein 1 (VMP1) is an essential autophagy protein. The expression of
相关内容
Research Focus: Autophagy
New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持