Tumor susceptibility gene 101 protein (TSG101), also known as ESCRT-I complex subunit TSG101, refers to a series of cytosolic protein complexes that are needed for the proper recognition and sorting of ubiquitin modified carrier proteins into the internal vesicles of the things like endosomes, and other multivesicular bodies (MVBs). TSG101 binds to the ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into MVBs. TSG101 also mediates the association between the ESCRT-0 and ESCRT-I complex in the passage of ubiquitin cargo proteins along the chain of ESCRTs. TSG101 interacts with a protein called stathmin, a cytoplasmic phosphoprotein that is implicated in tumorigenesis, and normal TSG101 appears to act as a negative regulator of growth, genetic stability and cell cycle regulation. Mutations and alternatively spliced mutants are associated with breast and other cancers suggesting that defects in TSG101 assist in tumorigenesis and progression.

TSG101 protein expressed in E. coli.

Anti-TSG101, clone 4A10 Antibody is an antibody against TSG101, clone 4A10 for use in Western Blotting, Immunohistochemistry, ELISA.

Immunohistochemistry Analysis (IHP): A 1:100 dilution from a representative lot detected TSG101 in colon carcinoma tissue.

ELISA: The specificity of a representative lot was confirmed by ELISA analysis.

Evaluated by Western Blotting in HeLa, NIH/3T3, JC, C2C12 , HEK293, A431 and BCL-1 cell lysates.

Western Blotting Analysis (WB): A 1:500-1,000 dilution of this antibody detected TSG101 in HeLa, NIH/3T3, JC, C2C12 , HEK293, A431 and BCL-1 cell lysates.

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