Anti-CD1d/KRN7000 Antibody, clone L363

Antigen-presenting glycoprotein CD1d1 (UniProt P11609; also known as CD1d.1, T-cell surface glycoprotein CD1d1) is encoded by the Cd1d1 (also known as Cd1.1, Ly-38, AI747460) gene (Gene ID 12479) in murine species. CD1d is a nonpolymorphic MHC class I-like molecule, which presents nonpeptide ligands (e.g. glycolipids) to Type 1 or invariant natural killer T (iNKT) cells. iNKT cells differ from conventional T cells in that they recognize glycolipid antigens presented by the nonclassical β2-microglobulin (β2m)-associated CD1d. Recognition of CD1d-presented glycolipids by iNKT cells elicits the rapid secretion of both T helper type 1- (TH1-) and T helper type 2- (TH2-) associated cytokines without the need for priming. Murine CD1d is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature CD1d with a large extracellular region (a.a. 22-305), a transmembrane domain (a.a. 306-326), and a short cytoplasmic tail (a.a. 327-336).

~38 kDa calculated

C-terminally BirA-His-tagged soluble mCD1d/mβ2m/KRN7000 complex cross-linked with purified protein derivative (PPD) from M. tuberculosis strain H37Ra.

Epitope: Extracellular domain

Flow Cytometry Analysis: A representative lot detected α-galactosylceramide-/αGC-complexed CD1d on murine MH-S alveolar macrophages, LA-4 lung adenoma, and JAWSII bone marrow-derived dendritic cells (Rizvi, Z.A., et al. (2015). Toxicol. In Vitro. 29(6);1275-82.
Flow Cytometry Analysis: A representative lot detected αGC C20:2- and αGC26:0-complexed CD1d on the subpopulation of splenocytes with high CD11c-expression from mice injected with the respective α-GalCer analogs (Arora, P., et al. (2014). Immunity. 40(1):105-116).
Immunoprecipitation Analysis: A representative lot immunoprecipitated monoglycosylceramides-complexed mCD1d from rat RBL-Cd1d cells stably expressing murine CD1d (Kain, L., et al. (2014). Immunity. 41(4):543-554).
Immunocytochemistry Analysis: A representative lot detected α-GalCer-complexed murine CD1d on the surface of rat RBL-Cd1d cells stably expressing murine CD1d by fluorescent immunocytochemistry (Kain, L., et al. (2014). Immunity. 41(4):543-554).
Immunohistochemistry Analysis: A representative lot detected αGC C20:2- and αGC26:0-complexed CD1d on splenocytes in frozen spleen sections from mice injected with the respective α-GalCer analogs (Arora, P., et al. (2014). Immunity. 40(1):105-116).
ELISA Analysis: A representative lot selectively reacted with KRN7000-/α-GalCer-, OCH-, or C20:2-complexed mCD1d, but not glycolipid-free mCD1d. Clone L363 does not react with iGb3-complexed mCD1d, KRN7000-complexed hCD1b or KRN7000-complexed hCD1d (Yu, K.O., et al. (2007). J. Immunol. Methods. 323(1):11-23).
Neutralizing Analysis: A representative lot blocked CD1d-expressing cells from stimulating DN32.D3 NKT cells (Kain, L., et al. (2014). Immunity. 41(4):543-554).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CD1d/KRN7000 Antibody, clone L363 is validated for use in Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and ELISA for the detection of CD1d/KRN7000.

Clone L363 selectively reacts with KRN7000-/α-GalCer-, OCH-, or C20:2- complexed mCD1d, but not glycolipid-free mCD1d. Clone L363 does not react with iGb3-complexed mCD1d, KRN7000-complexed hCD1b or KRN7000-complexed hCD1d (Yu, K.O., et al. (2007). J. Immunol. Methods. 323(1):11-23).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Flow Cytometry in alpha-GalCer glycolipid-treated RMA-S/mCD1d (WT) cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD1d/KRN7000 in alpha-GalCer glycolipid-treated RMA-S/mCD1d (WT) cells.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.