antibody product type
primary antibodies
biological source
mouse
antibody form
purified immunoglobulin
clone
L363, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Cd1D1(12479)
General description
Immunogen
Application
Flow Cytometry Analysis: A representative lot detected αGC C20:2- and αGC26:0-complexed CD1d on the subpopulation of splenocytes with high CD11c-expression from mice injected with the respective α-GalCer analogs (Arora, P., et al. (2014). Immunity. 40(1):105-116).
Immunoprecipitation Analysis: A representative lot immunoprecipitated monoglycosylceramides-complexed mCD1d from rat RBL-Cd1d cells stably expressing murine CD1d (Kain, L., et al. (2014). Immunity. 41(4):543-554).
Immunocytochemistry Analysis: A representative lot detected α-GalCer-complexed murine CD1d on the surface of rat RBL-Cd1d cells stably expressing murine CD1d by fluorescent immunocytochemistry (Kain, L., et al. (2014). Immunity. 41(4):543-554).
Immunohistochemistry Analysis: A representative lot detected αGC C20:2- and αGC26:0-complexed CD1d on splenocytes in frozen spleen sections from mice injected with the respective α-GalCer analogs (Arora, P., et al. (2014). Immunity. 40(1):105-116).
ELISA Analysis: A representative lot selectively reacted with KRN7000-/α-GalCer-, OCH-, or C20:2-complexed mCD1d, but not glycolipid-free mCD1d. Clone L363 does not react with iGb3-complexed mCD1d, KRN7000-complexed hCD1b or KRN7000-complexed hCD1d (Yu, K.O., et al. (2007). J. Immunol. Methods. 323(1):11-23).
Neutralizing Analysis: A representative lot blocked CD1d-expressing cells from stimulating DN32.D3 NKT cells (Kain, L., et al. (2014). Immunity. 41(4):543-554).
Apoptosis & Cancer
Apoptosis - Additional
Biochem/physiol Actions
Physical form
Preparation Note
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Flow Cytometry Analysis: 0.1 µg of this antibody detected CD1d/KRN7000 in alpha-GalCer glycolipid-treated RMA-S/mCD1d (WT) cells.
Other Notes
Disclaimer
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wgk
WGK 2
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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