Anti-DNA Ligase 3 Antibody, clone 7H6.1

GST-tagged recombinant protein corresponding to the C-terminal of human DNA Ligase 3.

~113/106 kDa observed. 112.9 kDa (isoform 1), 106.0 kDa (isoform 2), 102.7 kDa (isoform 3), 95.8 kDa (isoform 4) calculated.

Epitope: C-terminal

Expected to react with all four spliced isoforms.

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA Ligase 3 in 10 µg of HeLa nuclear extract.

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