Anti-SKI2W Antibody, clone 8D10.2

Helicase SKI2W (UniProt Q15477; also known as Helicase-like protein, HLP, Ski2) is encoded by the SKIV2L (also known as DDX13, SKI2W, SKIV2, THES2, W) gene (Gene ID 6499) in human. Ski2w is a mammalian homologue of yeast ski2 protein proposed to be involved in RNA processing. The presence of a DEVH-box motif (a.a. 423-426) and its in vitro ATPase/GTPase activity both point to Ski2w being an RNA helicase, although such biological activity has not been demonstrated in vivo. In addition, human Ski2w contains two consecutive leucine zipper motifs commonly involved in protein-protein interaction, as well as an RGD motif (a.a. 238-240) that can potentially interact with cell adhesion molecules. Ski2w is ~140 kDa in size and present in the nucleolus and in the cytoplasm. Ski2w is reported to be associated with ribosomes in an mRNA-independent manner, suggesting a role in protein synthesis regulation. SKIV2L gene mutations have been identified in patients with Tricohepatoenteric syndrome (THES) characterized by intrauterine growth retardation, severe diarrhea in infancy, facial dysmorphism, immunodeficiency, and hair abnormalities, mostly trichorrhexis nodosa.

~140 kDa observed. 137.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinant fragment from the C-terminal half of human SKI2W.

Anti-SKI2W, clone 8D10.2 Antibody, Cat. No. MABE1048, is a highly specific mouse monoclonal antibody that targets SKI2W and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SKI2W in human prostate cancer, pancreas, and skeletal muscle tissue sections.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SKI2W in 10 µg of HepG2 cell lysate.

Research Category
Epigenetics & Nuclear Function

Clone 8D10.2 targets an internal region C-terminal to the helicase domain.

Format: Purified

Protein G purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected SKI2W in 10 µg of HeLa cell lysate.

Concentration: Please refer to lot specific datasheet.

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