conjugate
unconjugated
antibody form
purified antibody
clone
4F7, monoclonal
purified by
using protein G
species reactivity
mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ChIP: suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant fragment corresponding to 191 amino acids from the C-terminal half of mouse Polycomb protein Suz12.
~85 kDa observed; 83.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Suz12 in Chromatin Immunoprecipitation applications (Isono, K., et. al. (2013). Dev Cell. 26(6):565-77).
Western Blotting Analysis: A representative lot detected Suz12 in Western Blotting applications (Endoh, M., et. al. (2008). Development. 135(8):1513-24).
Western Blotting Analysis: A representative lot detected Suz12 in Western Blotting applications (Endoh, M., et. al. (2008). Development. 135(8):1513-24).
Biochem/physiol Actions
Clone 4F7 is a mouse monoclonal antibody that detects Polycomb protein Suz12. It targets an epitope within the C-terminal region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in F9 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Suz12 in F9 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Suz12 in F9 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MABE1876-25UG | 04054839010415 |
| MABE1876-100UG | 04054839010408 |
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