Anti-phospho-ACD (Ser255) Antibody, clone 34B10

Adrenocortical dysplasia protein homolog (UniProt: A0A590TQL1; also known as TPP1, ACD) is encoded by the ACD (also known as hCG_27140) gene (Gene ID: 65057) in human. ACD (TPP1) is one of the shelterin components that is involved in both telomere protection and telomere length regulation. Shelterin is known to associate with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without this protection, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. ACD/TPP1 contains an oligosaccharide binding-fold (OB-fold) domain that provides an interface to recruit telomerase. Within its OB-fold domain is a cluster of amino acids known as the TEL patch (TPP1-Glu-Leu) that contains an interaction interface with telomerase. In the G2/M phase, it interacts with the cell cycle regulator kinase NEK6 that phosphorylates a highly conserved serine 255 that is located in the intervening region between the telomerase recruiting OB-fold and the POT1 (Protection Of Telomeres 1) recruiting domain. POT1 is reported to negatively regulate ACD/TPP1 phosphorylation. Phosphorylation of serine 255 is shown to be important in the interaction between ACD/TPP1 and telomerase. (Ref.: Henslee, G., et al. (2021). Cold Spring Harb. Mol. Case Stud. 7(1); a005454; Hirai, Y., et al. (2016). Genes Cells. 21(8); 874-889).

Clone 34B10 is a mouse monoclonal antibody that detects ACD (TPP1) phosphorylated on serine 255.

KLH-conjugated linear peptide corresponding to 19 amino acids surrounding phosphoserine 255 from the internal region of human ACD/TPP1.

Quality Control Testing

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ACD/TPP1 phosphorylated on serine 255 in lysates from HEK293T cells simultaneously transfected with HA-NEK6 and FLAG-TPP1 plasmids, but not in lysates from mock transfected cells (HEK293T/pcDNA3) or cells transfected only with FLAG-TPP1.

Tested Applications

Western Blotting Analysis: A representative lot detected ACD in Western Blotting applications (Hirai, Y., et al. (2016). Genes Cells. 21(8):874-89).

Immunocytochemistry Analysis: A representative lot detected ACD in Immunocytochemistry applications (Hirai, Y., et al. (2016). Genes Cells. 21(8):874-89).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-phospho-ACD (Ser255), clone 34B10, Cat. No. MABE1904, is a mouse monoclonal antibody that detects ACD (TPP1) phosphorylated on serine 255 and is tested for use in Immunocytochemistry and Western Blotting.

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.