MABE340
抗-MDM2抗体，克隆IF2
clone IF2, from mouse
别名:
E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
IF2, monoclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
human
Citations:
11
Technique(s):
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-MDM2抗体，克隆IF2, clone IF2, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
IF2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MDM2(4193)
Analysis Note
对照
A431细胞裂解物
A431细胞裂解物
通过蛋白质印迹法在A431细胞裂解物中进行评估。
蛋白质印迹分析：1 µg/mL该抗体在10 µg A431细胞裂解物中检测到MDM2。
蛋白质印迹分析：1 µg/mL该抗体在10 µg A431细胞裂解物中检测到MDM2。
Application
抗MDM2抗体，克隆IF2是用于检测MDM2（也称为E3泛素蛋白连接酶MDM2或Hdm2）的小鼠单克隆抗体，&已在WB&IHC中进行了验证。
研究子类别
细胞周期、DNA复制&修复
细胞周期、DNA复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白质印迹分析：一个代表性批次在Raji、HepG2和Molt-4细胞裂解物中检测到MDM2。
免疫组织化学分析：一个代表性批次以1:50稀释度在人前列腺癌组织中检测到MDM2。
免疫组织化学分析：一个代表性批次以1:50稀释度在人前列腺癌组织中检测到MDM2。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
MDM2是一种E3泛素连接酶，因其在调节p53肿瘤抑制因子中的作用而受到最广泛的研究。MDM2以两种主要方式拮抗p53的转录活性。MDM2的N末端相互作用并禁用p53的反式激活域。此外，MDM2的C末端E3连接酶区域可以将单泛素连接到p53，从而导致p53从细胞核转运到细胞质，从而阻止p53与靶DNA序列的相互作用。此外，MDM2也可能将多聚泛素链连接到p53，导致蛋白酶体降解p53。MDM2可能与Mdmx蛋白协同产生这些作用。总体而言，MDM2抑制p53诱导细胞周期停滞或凋亡的能力，并可能有助于肿瘤发生。
观测分子量~85 kDa和~60 kDa。Uniprot描述了12-56 kDa之间的许多亚型，它们可以高度磷酸化和泛素化。
Immunogen
对应于人MDM2的重组蛋白。
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG2bκ，溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
纯化蛋白G
Preparation Note
自接收之日起，在2-8°C下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Robert F H Walter et al.
Journal of oncology, 2018, 1986982-1986982 (2018-08-17)
Previously, our group demonstrated that nuclear expression of E3 ubiquitin ligase (MDM2) in malignant pleural mesothelioma (MPM) is significantly associated with decreased overall survival. A possible explanation may be that overexpression of MDM2 leads to a proteasomal degradation of TP53
Marcin Poreba et al.
Cell death and differentiation, 26(12), 2695-2709 (2019-04-13)
Most caspases can be positioned unambiguously within the regulated cell death networks of apoptosis and pyroptosis, but the role of caspase-2, a highly conserved protease within the family, remains enigmatic. This is mainly due to lack of selective chemical and
P Tanière et al.
British journal of cancer, 85(5), 721-726 (2001-09-05)
In Europe, high incidence rates of oesophageal squamous cell carcinoma (SCCE) are observed in western France (Normandy and Brittany) and in north-eastern Italy. Analysis of TP53 mutations in tumours from these regions has shown a high prevalence of mutations at
Eva Röijer et al.
The American journal of pathology, 160(2), 433-440 (2002-02-13)
Carcinoma ex pleomorphic adenoma (CexPA) is a carcinoma developing within a pre-existing benign pleomorphic adenoma (PA). Here we describe the identification and characterization of a series of genetic events leading to translocation, deletion/amplification, and overexpression of the HMGIC and MDM2
A Lakoma et al.
Cell death discovery, 1 (2015-01-01)
Neuroblastoma is an aggressive pediatric malignancy which is >98% p53 wild-type at diagnosis. As a primary repressor of p53 activity and part of a p53-activated negative feedback loop, targeting of mouse double minute 2 homolog (MDM2) is an attractive therapeutic
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