Anti-WDR5 Antibody, clone 7B11

WD repeat-containing protein 5 (WDR5) or alternatively BMP2-induced 3kb gene protein, is encoded by the human gene named WDR5/BIG3 and is part of a family of WD-40 repeat proteins that appear to facilitate the formation of multi-protein complexes much like a sticky tape will bind a variety of components together. The WD repeat proteins are involved in a wide array of cellular functions and processes including cell cycle progression, homeobox gene activation, apoptosis, gene regulation and histone methylation. One of WDR5 best studied activities is as part of a multi-protein complex that facilitates the methylation of histones, particularly histone H3, through its interaction with MLL a histone methyltransferase. WDR5 is also expressed in developing bone cells including osteoblasts, chondrocytes, and marrow stromal cells and works to accelerate osteoblast differentiation and positively promotes Wnt signaling in developing bone tissues. EMD-Millipore’s Anti-WDR5 mouse monoclonal antibody has been tested in western blot using HeLa cell lysates.

Purified recombinant fragment of human WDR5 expressed in E. Coli.

Anti-WDR5 Antibody, clone 7B11 is a highly specific mouse monoclonal antibody & that targets WDR5 & has been tested in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WDR5 in HeLa cell lysate.

Optimal working dilutions must be determined by end user.

~40 kDa observed

Unpurified

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa cell lysate

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