MABE455
Anti-CHD-4 Antibody, clone 3F2/4
clone 3F2/4, from mouse
别名:
Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F2/4, monoclonal
Application:
ChIP, IF, IHC, WB
Citations:
1
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F2/4, monoclonal
species reactivity
mouse, human
technique(s)
ChIP: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHD4(1108)
General description
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
~220 kDa observed
Immunogen
Linear peptide corresponding to human CHD-4.
Application
Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Wei Shi et al.
Development (Cambridge, England), 151(8) (2024-04-15)
Regulation of chromatin states is essential for proper temporal and spatial gene expression. Chromatin states are modulated by remodeling complexes composed of components that have enzymatic activities. CHD4 is the catalytic core of the nucleosome remodeling and deacetylase (NuRD) complex
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