MABE78
Anti-MiTF Antibody, clone 3D1.2
clone 3D1.2, from mouse
别名:
Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
产品名称
Anti-MiTF Antibody, clone 3D1.2, clone 3D1.2, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3D1.2, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MITF(4286)
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Application
Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
Research Category
Signaling
Signaling
Research Sub Category
Transcription Factors
Transcription Factors
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
Immunogen
GST-tagged recombinant protein corresponding to human MiTF.
Other Notes
Replaces: MAB3747
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% sodium azide, pH 7.1.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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