MABE914
Anti-Mi-2β Antibody, clone 16G4
clone 16G4, from mouse
别名:
Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
16G4, monoclonal
Application:
ChIP
western blot
western blot
Species reactivity:
human
Citations:
Technique(s):
ChIP: suitable (ChIP-seq)
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-Mi-2β Antibody, clone 16G4, clone 16G4, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
16G4, monoclonal
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHD4(1108)
Analysis Note
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in a Jurkat cell lysate.
Application
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was used for ChIP. See Zhang et al. (2012).
ChIP-seq: representative lot of this antibody was used for ChIP-seq. See Zhang et al. (2012).
ChIP-seq: representative lot of this antibody was used for ChIP-seq. See Zhang et al. (2012).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This Anti-Mi-2β Antibody, clone 16G4 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
~220 kDa observed
Immunogen
Recombinant protein corresponding to Human Mi-2β.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
10 - Combustible liquids
wgk
WGK 2
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