MABE915
Anti-Mi-2β Antibody, clone 2G8
clone 2G8, from mouse
别名:
Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
2G8, monoclonal
Conjugate:
unconjugated
Application:
ChIP
western blot
western blot
Species reactivity:
human
Citations:
Technique(s):
ChIP: suitable (ChIP-seq)
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-Mi-2β Antibody, clone 2G8, clone 2G8, from mouse
clone
2G8, monoclonal
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHD4(1108)
Analysis Note
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in 10 µg of a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in 10 µg of a Jurkat cell lysate.
Application
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP (see Zhang, J., et al 2012).
ChIP-seq: A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP-seq (see Zhang, J., et al 2012).
ChIP-seq: A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP-seq (see Zhang, J., et al 2012).
This Anti-Mi-2β Antibody, clone 2G8 is validated for use in Western Blotting and ChIP-seq and Culture Confirmation for the detection of Mi-2β.
General description
Chromodomain-helicase-DNA-binding protein 4 (CHD-4), often referred to as Mi-2β, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Mi-2β has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of Mi-2β.
~ 220 kDa observed
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Format: Purified
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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