MABE920
Anti-Mi-2β Antibody, clone 17H11
clone 17H11, from mouse, purified by affinity chromatography
别名:
Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
17H11, monoclonal
Application:
ChIP, WB
Citations:
-
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
17H11, monoclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq), western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHD4(1108)
General description
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
~ 220 kDa observed
Immunogen
Recombinant protein corresponding to Human Mi-2β (CHD-4).
Application
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.
ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.
ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histone Modifying Proteins
Chromatin Biology
Histone Modifying Proteins
This Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
Biochem/physiol Actions
Recognizes Mi-2β (CHD-4)
Physical form
Protein G Purified
Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in a Jurkat cell lystate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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