Hepatocyte nuclear factor 1-beta (UniProt P35680; also known as HNF-1-beta, HNF-1B, Homeoprotein LFB3, TCF-2, Transcription factor 2, Variant hepatic nuclear factor 1, vHNF1) is encoded by the HNF1B (also known as TCF2, VHNF1) gene (Gene ID 6928) in human. HNF-1-beta is involved in transcriptional and functional regulation of the liver and biliary system, as well as of the kidneys, urogenital tract and pancreatic β-cells. HNF-1-beta deficiency due to HNF1B mutations is a well known cause of maturity-onset diabetes of the young 5 (MODY5), also referred to as RCAD (renal cysts and diabetes) due to the frequent co-occurrence of renal cysts and/or other inborn urogenital abnormalities. HNF-1-beta is expressed in the gall bladder, liver and intrahepatic bile ducts of mouse embryos and in liver of adult mice. HNF-1-beta is shown to co-regulate morphogenesis of the biliary system and HNF1B knockout mice suffer from severe neonatal cholestasis and jaundice due to the abnormally developed gall bladder and dysfunctional intrahepatic bile ducts.

~61 kDa observed

Epitope: Internal (N-terminal half)

GST-tagged recombinant protein corresponding to the Internal (N-terminal half) of human HNF-1-beta.

Anti-HNF-1-beta Antibody, clone 12A5.1 is an antibody against HNF-1-beta for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected HNF1B in human kidney and rat liver tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Expected to react with isoform 1 (A) and isoform 3 (C). Reactivity toward isoform 2 (B) and isoform 4 has not been determined.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HNF1B in 10 µg of rat brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

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