Anti-CD45 (human), violetFluor^® 450, clone HI30 Antibody

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

This mouse monoclonal Anti-CD45 Antibody (human), violetFluor^® 450, clone HI30, Cat. No. MABF1611 is validated for use in Flow Cytometry, for the detection of CD45.

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 1 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.

Concentration: Please refer to lot specific datasheet.

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