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Merck
CN

MABF161B

Anti-CD45 (human), redFluor® 710, clone HI30 Antibody

clone HI30, from mouse, redFluor® 710

别名:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
redFluor® 710
Clone:
HI30, monoclonal
Application:
FACS
Citations:
-
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biological source

mouse

conjugate

redFluor® 710

antibody form

purified antibody

antibody product type

primary antibodies

clone

HI30, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

target post-translational modification

unmodified

Quality Level

Gene Information

human ... PTPRC(5788)

General description

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This mouse monoclonal Anti-CD45 Antibody (human), redFluor® 710, clone HI30, Cat. No. MABF1613 is validated for use in Flow Cytometry, for the detection of CD45.

Physical form

Purified by size-exclusion chromatography
Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

redFluor is a registered trademark of Tonbo Biotechnologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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