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Merck
CN

MABF2156

Anti-TLR9 Antibody, clone NaR9

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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conjugate

unconjugated

antibody form

purified antibody

clone

NaR9
monoclonal

purified by

using protein G

species reactivity

mouse, mouse, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG2aκ

UniProt accession no.

mode of action

| inhibits

target post-translational modification

unmodified

Analysis Note

Evaluated by Immunocytochemistry in Raw264.7 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TLR9 in Raw264.7 cells.

Application

Immunocytochemistry Analysis: A representative lot detected TLR9 in Immunocytochemistry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Flow Cytometry Analysis: A representative lot detected TLR9 in Flow Cytometry applications (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Inhibits Activity/Function: A representative lot inhibited TLR9-dependent cytokine production in vitro by bone marrow-derived macrophages and conventional dendritic cells. (Murakami, Y., et. al. (2017). Sci Rep. 7:44042).

Biochem/physiol Actions

Clone NaR9 is a mouse monoclonal antibody that specifically detects Toll-like receptor 9 in murine species. It targets an epitope within the N-terminal region.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

116.41 kDa calculated.
Ba/F3 cells expressing full-length mouse TLR9 protein. Immunization was carried out on Tlr9 -/- mice.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

法规信息

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