Anti-HLA-F, clone 3D11

Anti-HLA-F, clone 3D11, Cat. No. MABF2162, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain F and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

ELISA Flow Cytometry Immunohistochemistry (Paraffin) Western Blotting

ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).

Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).

Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).

Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).

Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.

Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HLA-F in human placenta tissue sections.

HLA class I histocompatibility antigen, alpha chain F (UniProt: P30511; also known as CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F) is encoded by the HLA-F (also known as HLA-5.4, HLAF) gene (Gene ID: 3134) in human. HLA-F is a single-pass type I membrane heterodimeric protein that is involved in the presentation of foreign antigens to the immune system. It is expressed in B cells, NK cells, monocytes, and T cells and its expression is reported to be coincident with the activated immune response. It is mainly localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off. The mature form has an extracellular domain (aa 22-305), a transmembrane domain (aa 306-329), and a short cytoplasmic domain (aa 330-346). The HLA-F locus has very low allelic polymorphism and is highly conserved in distantly related non-human primates indicating its conserved function. It is involved in the regulation of immune system in pregnancy, infection, and autoimmunity by signaling through NK cell receptors. (Ref.: Dulberger, CL et al. (2017). Immunity 46(6); 1018 1029; Lee, N., et al. (2010). Eur. J. Immunol. 40(8): 2308 2318).

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