偶联物
unconjugated
抗体形式
purified antibody
克隆
4
monoclonal
纯化方式
using protein G
种属反应性
mouse, virus
浓度
(Please refer to lot specific datasheet.)
技术
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
Recombinant 3D polymerase from Enterovirus 71.
~52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
应用
Immunofluorescence Analysis: A representative lot detected Enterovirus 71 3D in Immunofluorescnece applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Enterovirus 71 3D in Immunoprecipitation applications (Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Western Blotting Analysis: A representative lot detected Enterovirus 71 3D in Western Blotting applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, H.I., et. al. (2018). PLoS One. 13(1): e0191617).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Enterovirus 71 3D in Immunoprecipitation applications (Huang, P.N., et. al. (2017). PLoS Pathog. 13(5): e1006375).
Western Blotting Analysis: A representative lot detected Enterovirus 71 3D in Western Blotting applications (Huang, H.I., et. al. (2014). Virology. 468-470:592-600; Huang, H.I., et. al. (2018). PLoS One. 13(1): e0191617).
生化/生理作用
Clone 4 is a mouse monoclonal antibody that specifically detects Enterovirus 71 3D polymerase in EV71 infected cells.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Evaluated by Western Blotting in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Enterovirus 71 3D in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Enterovirus 71 3D in Enterovirus 71 infected human embryonal rhabdomyosarcoma cell lysates.
其他说明
Virus (Enterovirus)
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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