Anti-CD11a Antibody, clone TS2/4

Integrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are heterodimeric, integral membrane proteins composed of a variable -subunit and a conserved β-subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the -subunit. CD11a is a single-pass type I membrane glycoprotein that noncovalently associates with β2 integrin to forms a heterodimer and serve as a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. CD11a is considered as a pan-leukocyte marker and is expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils, and eosinophils. It is involved in generation of common lymphoid progenitor cells in bone marrow. In resting T-cells, about 40% of CD11a is constitutively phosphorylated and phosphorylation causes conformational changes needed for ligand binding and is considered to be essential for activation by some physiological agents. CD11a is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It has a large extracellular domain (aa 26-1090), a transmembrane domain (aa 1091-1111), and a cytoplasmic domain 1112-1170). Three isoforms of CD11a have been described that are produced by alternative splicing. (Ref.: Bose, TO., et al. (2014). J. Immunol. 193(6); 2863-2872; Fagerholm, SC., et al. (2005). J. Cell Biol. 171(4); 705-715; Sanchez-Madrid, F., et al. (1982). Proc. Natl. Acad. Sci. USA. 79(23); 4489-7493).

Human Leukocyte Antigen- DR isotype (HLA-DR) expressing cytotoxic T lymphocytes.

Quality Control Testing

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected CD11a/ITGAL in one million THP-1 cells.

Tested Applications

Immunoprecipitation (IP) Application: A representative lot immunoprecipitated CD11a in immunoprecipitation application (Sanchez-Madrid, F., et al. (1982). Proc. Natl. Acad. Sci. USA. 79(23); 7489-7493).

Gel Electrophoresis Application: A representative lot detected CD11a in Gel Electrophoresis application (Sanchez-Madrid, F., et al. (1982). Proc. Natl. Acad. Sci. USA. 79(23); 7489-7493).

Inhibition Assay: A representative lot inhibited CTL-mediated killing of Daudi cells. (Sanchez-Madrid, F., et al. (1982). Proc. Natl. Acad. Sci. USA. 79(23); 7489-7493).

Radioimmunoassay Application: A representative lot detected CD11a in Radioimmunoassay applications (Dustin, ML., et al. (1992). J. Immunol. 148(9); 2654-2663).

Immunoaffinity Purification: A representative lot was used for immunoaffinity purification of CD11a. (Dustin, ML., et al. (1992). J. Immunol. 148(9); 2654-2663).

Immunofluorescence Application: A representative lot detected CD11a in Immunofluorescence microscopy application. (Dustin, ML., et al. (1992). J. Immunol. 148(9); 2654-2663).

Flow Cytometry (FC) Application: A representative lot detected CD11a in Flow Cytometry application. (Sanchez-Madrid, F., et al. (1982). Proc Natl Acad Sci USA. 79(23):7489-93; Samuelsson, M., et al. (2017). Sci Signal. 10(509):eaai8629.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone TS2/4 is a mouse monoclonal antibody that detects CD11a/ITGAL It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.