Anti-BLAST-2 (CD23), clone EBVCS3

Activity Assay Flow Cytometry Immunofluorescence

Anti-BLAST-2 (CD23), clone EBVCS3, Cat. No. MABF2318, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tested for use in Activity Assay, Flow Cytometry, and Immunofluorescence.

Flow Cytometry Analysis: A representative lot detected BLAST-2 (CD23) in Flow Cytometry applications (Stohl, W., et. al. (1990). Cell Immunol. 130(2):257-70).

Activity Assay Analysis: A representative lot detected BLAST-2 (CD23) in Activity Assay applications (Schattner, E.J., et. al. (1996). Blood. 88(4):1375-82)

Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).

Clone EBVCS3 is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (CD23; BLAST-2).

EBV-transformed human cells.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Purified mouse monoclonal antibody IgM in PBS without preservatives.

Evaluated by Flow Cytometry in Raji B cells.

Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in Raji cells.

Low affinity immunoglobulin epsilon Fc receptor (UniProt: P06734; also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23) is encoded by the FCER2 (also known as CD23A, CLEC4J, FCE2, IGEBF) gene (Gene ID: 2208) in human. Epstein-Barr virus (EBV) is reported to transform human B lymphocytes and is a causative agent for Burkitt s lymphoma and infectious mononucleosis. EBV induces the expression of one or more antigens (e.g., CD23) on the surface of adult human B-lymphocytes soon after infection. CD23 is a homotrimeric, single-pass type II membrane protein that serves as a low-affinity receptor for Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in the differentiation of B-cells. CD23 can also exist as a soluble excreted form, sCD23 (aa 150-321), which is produced by ADAM10-mediated ectoderm shedding. CD23 has a cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-47), and an extracellular domain (aa 48-321). Clone EBVCS3 can specifically detect antigens that are present on the surface of EBV-transformed cells. These antigens are also recognized by autochthonous cytotoxic T cells specific for EBV-transformed cells. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.