conjugate
unconjugated
antibody form
purified antibody
clone
EBVCS4, monoclonal
purified by
(Ligatrap)
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable
isotype
IgMκ
UniProt accession no.
target post-translational modification
unmodified
General description
EBV-transformed human cells.
36.47 kDa calculated.
Application
Radioimmunoassay Analysis: A representative lot detected BLAST-2 (CD23) in Radioimmunoassay applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).
Immunoprecipitation Analysis: A representative lot immunoprecipitated BLAST-2 (CD23) in Immunoprecipitation applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).
Immunoprecipitation Analysis: A representative lot immunoprecipitated BLAST-2 (CD23) in Immunoprecipitation applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Biochem/physiol Actions
Clone EBVCS4 is a mouse monoclonal antibody that specifically detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) in human cells.
Physical form
Purified mouse monoclonal antibody IgM in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Flow Cytometry in Raji B cells.
Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in one million Raji cells.
Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in one million Raji cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.