Interleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).

Clone MH9A4 is a mouse monoclonal antibody that detects human Interleukin-9.

Ovalbumin-conjugated recombinant human Interleukin-9.

Quality Control Testing

Evaluated by ELISA with recombinant human IL-9.

ELISA Analysis:: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).

Tested Applications

Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3; Chang, H.C., et al. (2010). Nat Immunol.;11(6):527-34; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.