MABF288
抗Roquin-1/2抗体（克3F12）
clone 3F12, from rat
别名:
Roquin-1, Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F12, monoclonal
Application:
IP, WB
Citations:
4
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F12, monoclonal
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RC3H1(149041)
General description
Roquin-1 与Roquin-2的一致性为45%，两者功能相同，属于RNA 结合蛋白锌指家族。 Roquin-1/2是胞质RNA抑制蛋白，与选择性RNA特异性结合，并促进成熟mRNA的脱腺苷化及其降解。Roquin-1/2靶向HMGXB3、ICOS、IER3、NFKBID、NFKBIZ、 PPP1R10、TNF和许多更关键的RNA。特别值得一提的是，由于其对 ICSO 和γ-干扰素以及辅助性T细胞激活的影响，研究人员研究了Roquin-1/2作为全身性炎症反应的介导因子以及自体免疫疾病中适应性和先天免疫反应的控制因子的功能。 Roquin 1/2 广泛表达，并位于细胞质和/或P小体，以及细胞应激情况下的胞浆应激颗粒。
计算分子量为125 kDa
Immunogen
对应于人Roquin-1/2的重组蛋白。
Application
蛋白印迹分析：一个代表性批次在正常MEF中检测到Roquin-1 & Roquin-2，在Roquin-1 缺陷性MEF只检测到Roquin-2，在Roquin-1/2 缺陷性MEF中两者都没有检测到。该抗体也在小鼠脾脏、 淋巴结、胸腺、脑& 肺组织、CD4+ T 细胞和胸腺细胞裂解液中检测到Roquin-1 和Roquin-2（Vogel, K.U., et al. (2013).Immunity.38:655-668）。
免疫沉淀分析：一个代表性批次使MEF细胞裂解液中的Roquin-1/2免疫沉淀（Vogel, K.U., et al. (2013).Immunity.38:655-668）。
免疫沉淀分析：一个代表性批次使MEF细胞裂解液中的Roquin-1/2免疫沉淀（Vogel, K.U., et al. (2013).Immunity.38:655-668）。
该抗-Roquin-1/2抗体（克隆3F12 ）经过验证可用于蛋白质印迹和免疫沉淀中检测Roquin-1/2。
Biochem/physiol Actions
一个代表性批次检测到Roquin-1 & Roquin-2（Vogel, K.U., et al. (2013).Immunity.38:655-668）。
Physical form
形式：纯化
Other Notes
浓度：请参考特定批次的数据表。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Johannes Braun et al.
Nucleic acids research, 46(22), 12109-12125 (2018-10-09)
Post-transcriptional gene regulation controls the amount of protein produced from a specific mRNA by altering both its decay and translation rates. Such regulation is primarily achieved by the interaction of trans-acting factors with cis-regulatory elements in the untranslated regions (UTRs)
Gregory J Connell et al.
RNA (New York, N.Y.), 29(8), 1117-1125 (2023-05-10)
The interactions of iron regulatory proteins (IRPs) with mRNAs containing an iron-responsive element (IRE) maintain cellular iron homeostasis and coordinate it with metabolism and possibly cellular behavior. The mRNA encoding transferrin receptor-1 (TFRC, TfR1), which is a major means of
Jaewoo Choi et al.
Cell cycle (Georgetown, Tex.), 17(18), 2243-2255 (2018-09-14)
Protein phosphorylation regulates a variety of cellular signaling pathways and fundamental mechanisms in cells. In this paper, we demonstrate that the mRNA decay factor Roquin2 is phosphorylated at tyrosine residue in position 691 in vivo. This phosphorylation disrupts the interaction
Jaewoo Choi et al.
Nature cell biology, 20(5), 586-596 (2018-04-27)
Kelch-like protein 6 (KLHL6) is an uncharacterized gene mutated in diffuse large B-cell lymphoma (DLBCL). Here we report that KLHL6 assembles with cullin3 to form a functional cullin-RING ubiquitin ligase. Mutations in KLHL6 inhibit its ligase activity by disrupting the
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