Anti-G-CSF-R/CD114 Antibody, clone LMM741

Granulocyte colony-stimulating factor receptor (UniProt: Q99062; also known as G-CSF receptor, G-CSF-R, CD114) is encoded by the CSF3R (also known as CSF3R) gene (Gene ID: 1441) in human. CD114/G-CSF-R belongs to the type I subgroup of the superfamily of cytokine receptors. G-CSF-R mediates granulocyte proliferation and differentiation in both normal and malignant hematopoiesis. G-CSF-R functions as the receptor for granulocyte colony-stimulating factor (G-CSF) and is activated via ligand-induced homodimerization. G-CSF-R is a single-pass type I membrane protein produced with a signal peptide sequence (aa 1-24), the removal of which yields the mature single transmembrane (aa 628-650) receptor with a C-terminal cytoplasmic tail (aa 651-836) and a large N-terminal extracellular domain (aa 25-627) composed of a C2-type Ig-like domain (aa 25-117) and five type-III fibronectin domains (aa 125-623). It is highly expressed in bone marrow cells, placenta, lung, lymphoid tissue and peripheral blood granulocytes. The G-CSF/GCSF-R complex mediates survival, proliferation, and maturation of neutrophils and regulates granulopoiesis under both normal and stress conditions. Cell signaling via G-CSF-R is transduced primarily via the Jak/STAT pathway, but the Ras/Raf/MAP kinase and PKB/Akt pathways are also involved. CSF3R gene mutations are well documented in congenital neutropenia as well as the associated predisposition to myelodysplasia and acute myeloid leukemia myeloproliferative disorders. (Ref.: Nicholson, S.E., et al. (1994). Proc Natl Acad Sci USA. 91(8):2985-8; Layton, J.E., et al. (1999). J Biol Chem. 274(25):17445-51).

CHO-6A11 cells transfected with full length human G-CSF-R/CD114.

Quality Control Testing

Evaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis(FC): 1.0 ug from a representative lot detected G-CSF-R/CD114 in human peripheral blood mononuclear cells (PBMC).



Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated G-CSF-R/CD114 in immunoprecipitation application (Nicholson, S.E., et al. (1994). Proc Natl Acad Sci USA. 91(8):2985-8).

Flow Cytometry Analysis: A representative lot detected G-CSF-R/CD114 in flow cytometry application (Layton, J.E., et al. (1999). J Biol Chem. 274(25):17445-51).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone LMM741 is a mouse monoclonal antibody that specifically detects G-CSF-R/CD114. It targets an epitope within the extracellular domain.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.