Complement C3 (UniProt: P01024; also known as C3, PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID: 718) in human. C3 is a secreted protein that plays a key role in the activation of the complement system. It s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. C3 has an anaphylatoxin-like domain (aa 693-728) and a NTR (Netrin) domain (aa 1518-1661). IC3 precursor is first processed by the removal of four arginine residues, forming two chains, β and , linked by a disulfide bond. C3 convertase between residues 748 and 749 activates C3 by cleaving the chain, releasing C3a anaphylatoxin and generating C3b. C3a appears to be important in many inflammatory responses. The active C3b fragment covalently binds to the cell or bacterial surface carbohydrates or immune aggregates, via its reactive thioester, and plays a role in opsonization. Surface-associated C3b generated in the classical pathway can also feed into the alternative pathway because it has the affinity for binding with the Factor B to form a pro-convertase C3bB. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, it acts as a chemoattractant for neutrophils. Defects in C3 gene can cause complement component 3 deficiency that leads to recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Defects in C3 gene are also known to cause age-related macular degeneration and hemolytic uremic syndrome that can lead to hemolytic anemia and renal failure Clone 10C7 specifically detects human and mouse C3b. (Ref.: Sharma, S., et al. (2020). Front. Immunol. 11; 462; Whipple, EC., et al. (2007). Mol. Immunol 44(4); 377-388).

Purified complement C3 from human serum.

Quality Control Testing

Evaluated by ELISA with purified native Human Complement C3

ELISA Analysis: Serial dilutions of this antibody detected Human Complement C3, but not Complement C4 protein.

Tested Applications

ELISA Analysis: A representative lot detected Complement C3/C3b in ELISA applications (Whipple, E.C., et al. (2007). Mol Immunol. 44(4):377-88).

Flow Cytometry Analysis: A representative lot detected Complement C3/C3b in Flow Cytometry applications (Sharma, S., et al. (2020). Front Immunol.11:462).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 10C7 is a mouse monoclonal antibody that detects Complement C3b.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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