biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
15A7.1, monoclonal
species reactivity
human, rat, mouse
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG2aκ
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ELAVL4(1996)
General description
Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.
~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human HuC/HuD.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.
Physical form
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human brain tissue lysate
Human brain tissue lysate
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MABN153 | 04053252858307 |