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Merck
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MABN1547

Anti-CASK Antibody, clone 2G5.1

clone 2G5.1, from mouse

别名:

Peripheral plasma membrane protein CASK, hCASK, Calcium/calmodulin-dependent serine protein kinase, Protein lin-2 homolog, CASK

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2G5.1, monoclonal
Application:
immunohistochemistry
western blot
Species reactivity:
human, rat
Citations:
1
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
O14936

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产品名称

Anti-CASK Antibody, clone 2G5.1, clone 2G5.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2G5.1, monoclonal

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_003679

UniProt accession no.

O14936

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CASK(8573)
rat ... Cask(29647)

Physical form

Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

General description

Peripheral plasma membrane protein CASK (EC 2.7.11.1; UniProt O14936; also known as Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, hCASK, Protein lin-2 homolog, Trinucleotide repeat containing 8) is encoded by the CASK (also known as CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8) gene (Gene ID 8573) in human. CASK is a membrane-associated guanylate kinase (MAGUK) family member involved in the regulation of cytoskeletal remodeling and cell migration. It consists of a Ca2+-calmodulin kinase, a PDZ, an SH3, and a guanylate kinase domain. CASK localizes at cell–cell adhesion sites, including synaptic junctions, epithelial junctions, and tight junctions, where it is reported to associate with other cell adhesion molecules, such as junctional adhesion molecule (JAM), cadherins, catenins, and syndecan-2. CASK and syndecan-2 expressions are upregulated in colorectal cancer and their upregulation significantly correlates with advanced tumor stage, lymphatic invasion, lymph node metastasis, vascular invasion, liver metastasis, and unresectable metastatic CRC.
~115 kDa observed

Analysis Note

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CASK in 10 µg of rat brain tissue lysate.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CASK in human kidney cortex tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-CASK Antibody, clone 2G5.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CASK.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Internal (C-terminal half)
GST-tagged recombinant protein corresponding to an internal fragment within the C-terminal half of human CASK.

Other Notes

Concentration: Please refer to lot specific datasheet.
Replaces: MAB5230

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Adeline Beuriot et al.
Heart rhythm, 17(5 Pt A), 786-794 (2020-01-07)
Membrane-associated guanylate kinase proteins function as adaptor proteins to mediate the recruitment and scaffolding of ion channels in the plasma membrane in various cell types. In the heart, the protein calcium/calmodulin-dependent serine protein kinase (CASK) negatively regulates the main cardiac

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White Paper: Further considerations of antibody validation and usage.

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