Vitamin D3 receptor (UniProt P11473; also known as 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1, VDR) is encoded by the VDR (also known as NR1I1) gene (Gene ID 7421) in human. VDR is a member of the superfamily of nuclear receptors for steroid hormones and acts as a ligand-inducible transcription factor that targets vitamin D responsive elements. Upon ligand binding, VDR complexes with the retinoid-X receptor (RXR), the heterodimer then targets hormone-response elements, resulting in transcription or transrepression of specific genes. Ligand binding also induces a conformational change at the C-terminal ligand-binding domain (LBD; a.a. 192-427), resulting in recruitment of the p160/SRC family coactivators whose acetylase activity plays an important role in transcription modulations via chromatin remodeling. VDR gene mutations are associated with type II vitamin D-resistant rickets and alopecia (hair loss).

~50 kDa observed. 48.29/53.88 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

GST-tagged recombinant fragment corresponding to the N-terninal of human vitamin D3 receptor.

Anti-Vitamin D3 Receptor Antibody, clone 6F3.1, Cat. No. MABN1601, is a highly specific mouse monoclonal antibody that targets Vitamin D3 receptor and has been tested in Western Blotting.

Research Category
Neuroscience

Clone 6F3.1 targets an epitope present at the N-terminal half of both spliced isoforms of human vitamin D3 receptor reported by UniProt (P11473).

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected vitamin D3 receptor in 10 µg of T47D human breast cancer cell lysate.

Concentration: Please refer to lot specific datasheet.

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