Anti-Catenin Delta-2 Antibody, clone 12B4

Catenin delta-2 (UniProt: Q9UQB3; also known as Delta-catenin, GT24, Neural plakophilin-related ARM-repeat protein, NPRAP, Neurojungin) is encoded by the CTNND2 (also known as NPRAP) gene (Gene ID: 1501) in human. Delta Catenin is a component of the cadherin-catenin cell adhesion complex and is enriched in synaptosomes. Catenin Delta 2 is a member of the beta-catenin superfamily and is expressed in the brain where it is required for normal cognitive development and its highest expression is observed in fetal brain. Catenin Delta 2 is phosphorylated by Cdk5 and by GSK-3beta. Catenin Delta 2 plays a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses. It is involved in the regulation of Wnt signaling where it acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3beta, phosphorylation, ubiquitination, and degradation. When bound to ZBTB33 (Zinc Finger and BTB Domain Containing 33), it functions as a transcriptional activator. The loss of CTNND2 gene has been associated with the intellectual disability observed in the cri du chat syndrome that is characterized by slow growth, microcephaly, delayed acquisition of psychomotor skills and intellectual disability. (Ref.: Yuan, L et al. (2015). J. Biol. Chem. 290(17):10947-57).

Clone 12B4.1 specifically detects CTNND2 in rat brain microsomal preparations. This clone targets a sequeence within 104 amino acids in the C-terminal half of CTNND2.

GST/His-tagged recombinat fragment corresponding to 104 amino acids from the C-terminal half of human CTNND2.

Anti-Catenin Delta-2 Antibody, clone 12B4, Cat. No. MABN2254, is a highly specific mouse monoclonal antibody that targets CTNND2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Catenin Delta-2 in human cerebellum, human cerebral cortex, rat hippocampus, and rat cerebral cortex tissues.

Research Category
Neuroscience

Evaluated by Western Blotting in rat brain microsomal prep tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Catenin Delta-2 in 10 µg of rat brain microsomal preparations.

~136 kDa observed; 132.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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