Delta-like protein 1 (UniProt: Q61483; also known as Drosophila Delta homolog 1, Delta1, DLL1) is encoded by the Dll1 gene (Gene ID: 13388) in murine species. DLL1 is a single-pass transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner. Following transinteraction, ligand cells produce mechanical force that promotes Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling. Delta1 is required for embryonic development and maintenance of adult stem cells in many different tissues. DLL1-induced Notch signaling plays a role in the regulation of cell lineage, cell specification, cell patterning, and morphogenesis through effects on differentiation and proliferation. DLL1 is reported to play a role in regulating neuronal differentiation of neural precursor cells via cell-cell interaction. DLL1 is reported to be co-expressed with DLL3 in the presomtic mesoderm of mouse embryo, however despite their co-expression, mutations in these proteins cause strikingly different effects. Heterozygous DLL1 mutant mice are lighter and smaller, with altered fat to lean ratio and display increased blood pressure and a slight bradycardia. They also exhibit reduced cholesterol and triglyceride levels in blood.

Clone PGPM-1F9 specifically detects murine Delta Like protein 1. It targets an epitope within 17 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of murine Delta-like protein 1.

Anti-Delta-like protein 1 antibody, clone PGPM-1F9, Cat. No. MABN2284, is a highly specific rat monoclonal antibody that targets Delta-like protein 1 and has been tested in Flow Cytometry, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Delta-like protein 1 in Immunohistochemistry applications (Bone, R.A., et. al. (2014). Development. 141(24):4806-16).

Flow Cytometry Analysis: 1 µg from a representative lot detected Delta-like protein 1 in one million DLL1-flag A7A1/CHO cells expressing DLL1.

Western Blotting Analysis: A representative lot detected Delta-like protein 1 in Western Blotting applications (Geffers, I., et. al. (2007). J Cell Biol. 178(3):465-76).

Research Category
Neuroscience

Evaluated by Western Blotting in DLL1-FLAG/CHO cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Delta-like protein 1 in 10 µg of lysate from CHO cells stably expressing Flag-tagged Delta-like Protein 1.

~78 kDa observed; 78.45 calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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