biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5H5.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ZMIZ1(57178)
General description
Retinoic acid-induced protein 17 (RAI17) is also called Zinc finger MIZ domain-containing protein 1 (ZMIZ1), and PIAS-like protein Zimp10. Retinoic acid-induced protein 17 promotes AR sumoylation and increases AR ligand-dependent transcriptional activity. Retinoic acid-induced protein 17 is highly expressed in ovary and at lower levels in prostate, spleen and testis.
~115 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~115 kDa and ~107 kDa
Immunogen
Recombinant protein corresponding to human RA-induced protein 17.
Application
Detect Retinoic acid-induced protein 17 using this mouse monoclonal antibody, Anti-RA-induced protein 17 Antibody, clone 5H5.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RA-induced protein 17 in human thalamus tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RA-induced protein 17 in 10 µg of SH-SY5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RA-induced protein 17 in 10 µg of SH-SY5Y cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Sameer Aryal et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(18) (2021-04-29)
Loss of the fragile X mental retardation protein (FMRP) causes fragile X syndrome (FXS). FMRP is widely thought to repress protein synthesis, but its translational targets and modes of control remain in dispute. We previously showed that genetic removal of
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| 货号 | GTIN |
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