Anti-MNR2/HB9 Antibody, clone 81.5C10

Homeodomain protein (UniProt: Q9YHY8) is encoded by the MNR2 gene (Gene ID: 395769) in chicken. MNR2 is a member of an evolutionarily conserved subgroup of Mnx class HD proteins that are characterized by a conserved homeodomain of about 60 amino acids. This domain facilitates DNA binding, allowing the protein to interact with specific regulatory regions of target genes. In vertebrates, MNR2 is specifically expressed in all somatic motoneurons and is necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. It is expressed by all motor neuron progenitors, but its expression is rapidly switched off in many postmitotic motor neurons. The onset of MNR2 expression is shown to occur at the time that motor neuron progenitors attain independence from Shh signaling. It is induced rapidly by Shh, prior to the expression of other somatic motor neuron transcription factors. MNR2 appears to regulate motor neuron identity by acting as a transcriptional repressor, indicating its key role of transcriptional repression in motor neuron specification. Clone 81.5C10 is suitable for use in multiple applications, including flow cytometry, immunohistochemistry, immunocytochemistry, and immunofluorescence and displays reactivity in multiple species. (Ref.: Ng, S-Y., et al. (2015). Cell Stem Cell. 17(5):569-584; William, CM., et al. (2003). Development. 130(8); 1523-1536; Tanabe, Y., et al. (1998). Cell. 95(1); 67-80).

GST-tagged recombinant chicken MNR2 protein expressed in E. coli.

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis(ICC): A 1:250 dilution of this antibody detected MNR2/HB9 in MCF-7 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected MNR2/HB9 in Immunofluorescence application (Montpetit, A., et al. (2008). PLoS Genet. 4(12):e1000296; Catlett, T.S., et al. (2021). Nat Commun. 12(1):2589; Liau, E.S., et al. (2023). Nat Commun. 14(1):46).

Immunocytochemistry Analysis: A representative lot detected MNR2/HB9 in Immunocytochemistry application (Montpetit, A., et al. (2008). PLoS Genet. 4(12):e1000296).

Flow Cytometry Analysis: A representative lot detected MNR2/HB9 in Flow Cytometry application (Ng, S-Y., et al. (2015). Cell Stem Cell. 17(5):569-84).

Immunohistochemistry Analysis: A representative lot detected MNR2/HB9 in Immunohistochemistry application (Cayuso, J., et al. (2006). Development. 133(3):517-28; Montpetit, A., et al. (2008). PLoS Genet. 4(12):e1000296; Liau, E.S., et al. (2023). Nat Commun. 14(1):46).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 81.5C10 is a mouse monoclonal antibody that specifically detects an epitope within the C-terminal region of MNR2/HB9.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.