Anti-Islet-1 homeobox Antibody, clone 40.2D6

Insulin gene enhancer protein ISL-1 (UniProt: P61374; also known as Islet-1) is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 is a DNA-binding transcriptional activator that is expressed in pancreatic islets and is essential for the maturation, proliferation, and survival of the endocrine pancreas. It recognizes and binds to the consensus octamer binding site 5′-ATAATTAA-3′ in promoter of target genes. Its DNA binding region is localized in amino acids 181-240. It also contains two LIM Zinc-binding domains (aa 17-79 and 90-133) and a LIM-binding domain (aa 262-291). It plays a key role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. It is reported to be essential for cardiac development and is considered as a marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells. It also controls the expression of fibroblast growth factor and bone morphogenetic protein. Islet-1 expression is a reliable marker for pancreatic endocrine tumors and their metastases. Two isoforms of islet-1 have been described that are produced by alternative splicing. (Ref.: Schmitt, AM., et al. (2008). Am. J. Surg. Pathol. 32(3); 420-425; Karlsson, O., et al. (1990). Nature. 344(6269); 879-882).

A recombinant fragment corresponding to 172 amino acids from the C-terminal half of rat Insulin gene enhancer protein ISL-1.

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Islet-1 homeobox protein in Human pancreas tissue sections.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Hoeber, J., et al. (2015). Sci Rep. 5:10666; Fischer, A.J., et al. (2010). PLoS One. 5(6):e10774; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17; Wan, Y., et al. (2019). Cell. 179(2):355-372.e23).

Immunocytochemistry Analysis: A representative lot detected Islet-1 homeobox in Immunocytochemistry applications (Vahsen, B.F., et al. (2022). Sci Rep. 12(1):12606; Hoeber, J., et al. (2015). Sci Rep. 5:10666).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Du, A., et al. (2009). Diabetes. 58(9):2059-69; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Islet-1 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes.58(9):2059-69).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Islet-1 homeobox in Immunoprecipitation applications (Velasco, S., et al. (2017). Cell Stem Cell. 20(2):205-217).

Western Blotting Analysis: A representative lot detected Islet-1 homeobox in Western Blotting applications (Haremaki, T., et al. (2010). Dev Dyn. 239(7):1977-87).

Electrophoretic Mobility Shift Assay (EMSA): A representative lot detected Islet-1 homeobox in Electrophoretic Mobility Shift Assay applications (Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17).

Flow Cytometry Analysis: A representative lot detected Islet-1 homeobox in Flow Cytometry applications (Meng, X., et al. (2020). Genes (Basel). 11(3):328).

Immunofluorescence Analysis: A representative lot detected Islet-1 homeobox in Immunofluorescence applications (Vahsen, B.F., et al. (2022). Sci Rep. 12(1):12606; Hoeber, J., et al. (2015). Sci Rep. 5:10666; Du, A., et al. (2009). Diabetes. 58(9):2059-69; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 40.2D6 is a mouse monoclonal antibody that detects Insulin gene enhancer protein ISL-1. It targets an epitope within the C-terminal half.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.