Anti-EVX1/2 Antibody, clone 99.1-3A2

Homeobox even-skipped homolog protein 1 (UniProt: P23683; also known as EVX-1) is encoded by Evx1 gene (Gene ID: 14028) in mouse. It belongs to a small family of vertebrate homeobox genes, comprised of EVX-1 and EVX-2, that have been shown to play an important role as transcriptional repressors during embryogenesis, and is induced by the presence of the FGF-4 growth factor. Members of the homeobox gene family are characterized by a conserved homeodomain that typically comprises around 60 amino acids. This domain allows it to bind DNA and regulate gene expression. EVX-1 is predominantly localized in the nucleus, and its nuclear localization is essential for its role in gene regulation during development. It may also play a role in the specification of neuronal cell types. Expression of the EVX-1 gene during embryogenesis defines the V0 and V1 classes of ventral interneurons in the ventral spinal cord, proposing that EVX-1 may also serve as a potential drug target for patients who suffer from loss of locomotive coordination due to spinal cord injury or stroke. Studies also indicate that both EVX-1 and EVX-2 are also involved in controlling body plan formation, as well as play a crucial role in gastrulation, appendage and tailbud development, and the specification of other neuronal cell types. Research shows that EVX1 is frequently hypermethylated in prostate cancer and predicts PSA recurrence. (Ref.: Pierani. A., et al. (1999). Cell. 97(7):903-915; Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40; Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316; Truong, M., et al. (2012). British Journal of Cancer. 107:100-107).

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of mouse EVX-1.

Quality Control Testing

Evaluated by Immunocytochemistry in NTERA-2 cells

Immunocytochemistry Analysis (ICC): A 1:50 dilution from a representative lot detected EVX1/2 in NTERA-2 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected EVX1/2 in Immunofluorescence application (Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).

Immunocytochemistry Analysis: A representative lot detected EVX1/2 in Immunocytochemistry application (Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316).

Immunohistochemistry Analysis: A representative lot detected EVX1/2 in Immunohistochemistry application (Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 99.1-3A2 is a mouse monoclonal antibody that specifically detects EVX1/2. It targets an epitope within 10 amino acids from the C-terminal region.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.