The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003).

~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms.

This antibody recognizes Neuregulin-1 at the extracellular domain.

Epitope: Extracellular domain

His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain.

Detect Neuregulin-1 using this Anti-Neuregulin-1 Antibody, clone D11 validated for use in WB & IC.

Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.)

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain microsomal tissue lysate

Evaluated by Western Blot in mouse brain microsomal tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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