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Merck
CN

MABN669

Sigma-Aldrich

Anti-Dopamine Transporter Antibody, clone mAb16

clone mAB16, from mouse

别名:

Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

mAB16, monoclonal

种属反应性

mouse, rat

技术

western blot: suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

mouse ... Slc6A3(13162)
rat ... Slc6A3(24898)

一般描述

The transmembrane dopamine transporter (DAT) is located on the presynaptic nerve terminal and is responsible for terminating dopaminergic transmission by transporting dopamine from the synaptic cleft into the dopaminergic neuron (reuptake). Dopaminergic pathways have been strongly implicated in reward and addiction, motivation, alcoholism, ADHD and degenerative motor diseases such as Parkinson′s, Huntington′s and Chorea. EMD Millipore’s Anti-Dopamine Transporter antibody has been validated in western blotting for mouse and rat specimens.
~ 68 kDa observed

免疫原

Epitope: Cytoplasmic domain
Linear peptide corresponding to the cytoplasmic domain of rat Dopamine Transporter.

应用

Anti-Dopamine Transporter Antibody, clone mAb16 is a highly specific mouse monoclonal antibody & that targets Dopamine Transporter & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Western Blotting Analysis: A representative lot from an independent laboratory detected Dopamine Transporter in rat striatal membrane tissue lysate.

生化/生理作用

This antibody recognizes the cytoplasmic domain of Dopamine Transporter.

外形

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

制备说明

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate
Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dopamine Transporter in 10 µg of mouse brain tissue lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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Jon D Gaffaney et al.
Neurochemistry international, 73, 16-26 (2013-11-26)
Ligand-induced changes in the conformation of extracellular loop (EL) 2 in the rat (r) dopamine transporter (DAT) were examined using limited proteolysis with endoproteinase Asp-N and detection of cleavage products by epitope-specific immunoblotting. The principle N-terminal fragment produced by Asp-N
Durairaj Ragu Varman et al.
Behavioural brain research, 408, 113267-113267 (2021-04-02)
Dopamine (DA) transporter (DAT) is dynamically regulated by several protein kinases and the Thr53 phosphorylation of DAT (pT53-DAT) is documented in heterologous cell models and in rat brain. However, the role of endogenous pT53-DAT in living animals has never been
Katrina L Paumier et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 40(4), 874-883 (2014-10-01)
In addition to alleviating depression, long-term adaptive changes induced by antidepressants may regulate neural plasticity in the diseased brain, providing symptomatic and disease-modifying effects in Parkinson's disease. The present study investigated whether chronic treatment with a frequently prescribed tricyclic antidepressant

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