Anti-Derlin-1 Antibody, clone 8G11.2

Derlin-1 (UniProt Q9BUN8; also known as Degradation in endoplasmic reticulum protein 1, Der1-like protein 1, DERtrin-1) is encoded by the DERL1 (also known as DER1) gene (ORF UNQ243/PRO276; Gene ID 79139) in human. Derlins are integral ER membrane proteins that form homo-oligomers and heteromultimeric complexes with membrane-bound machinery, including the E3 ubiquitin ligase Hrd1 and its binding partner SEL1L, involved in ER-associated degradation (ERAD). Before ERAD substrates can be processed by the cytoplasmic ubiquitin-proteasome system, they need to be translocated across the ER membrane through an AAA ATPase p97/VCP-driven process. Derlins are inactive members of the rhomboid family of intramembrane proteases that mediate the recruitment of AAA ATPase p97/VCP through interaction via their C-terminal SHP boxes. Derlin-1 is proposed to contain four transmembrane segments (a.a. 23-43, 60-80, 106-126, 155-175), three cytoplasmic domains (a.a. 23-43, 60-80, 106-126, 155-175), and two lumenol (a.a. 44-59 & 127-154) domains.

~24 kDa observed. 28.80 kDa (isoform 1) and 26.42 kDa (isoform 2) calculated.

Epitope: C-terminal cytoplasmic domain.

GST-tagged recombinant human derlin-1 C-terminal cytoplasmic fragment.

Immunocytochemistry Analysis: An 1:50 dilution from a representative lot detected Derlin-1 in HUVECs, A431 and HeLa cells.

Research Category
Signaling

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-Derlin-1 Antibody, clone 8G11.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of Derlin-1.

Expected to react with both spliced isoforms of human Derlin-1 reported by UniProt (Q9BUN8).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Derlin-1 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

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