Progesterone receptor (UniProt P06401; also known as Nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are also reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.

~110 kDa observed

Epitope: N-terminal

Recombinant protein corresponding to the N-terminal of human Progesterone Receptor B.

Immunocytochemistry Analysis: A representative lot stained formalin-fixed and paraffin-embedded MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed and Triton X-100-fixed MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoform B (PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in PR-A-transfected or untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Progesterone Receptor B Antibody, clone 250/H11 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor B.

Clone 250/H11 reacts with isoform 1 (isoform B; PR-B), but not isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in human breast cancer T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected progesterone receptor isoforms B (PR-B), but not isoform A (PR-A) in human breast cancer T47D cell lysate.

Concentration: Please refer to lot specific datasheet.

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