生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
22C2, monoclonal
种属反应性
human
种属反应性(根据同源性预测)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... MTOR(2475)
一般描述
The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.
免疫原
Linear peptide corresponding to human mTOR/FRAP.
应用
Detect the mTOR/FRAP protein using this Anti-mTOR Antibody, clone 22C2, validated for use in WB & IP.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
Western Blot Analysis: A representative lot was used by an independent laboratory in neuroblastoma cells. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)
质量
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate.
目标描述
~290 kDa observed
联系
Replaces: 04-385
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Jurkat cell lysate
Jurkat cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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