Anti-PTP1B Antibody, clone FG6-1G

PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

~50 kDa observed

Epitope: Catalytic domain

Recombinant protein corresponding to the catalytic domain of human PTP1B.

Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).

This antibody recognizes the catalytic domain of PTP1B.

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
TF-1 cell lysate

Evaluated by Western Blot in TF-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1140

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